Antibacterial and Haemolytic Activities of Tissue Proteins of the Major Carp, Labeo

The Antimicrobial proteins(AMPs) of kidney, liver, heart and gills of Labeo rohita were partially purified by acid extraction and ammonium sulphate precipitation. The proteins extracted from the tissues were estimated and found to be 106.5 mg, 64.0 mg, 160.0 mg and 48.2 mg/g.wet.wt respectively. Out of the four organs, the heart tissue has yielded the highest amount of protein i,e 160 mg /g wet .wt. All the four proteins exhibited bactericidal effects for most of the test organisms. The maximum diameter of inhibitory zone (11 mm) was observed for the kidney protein against E. coli and P. aeruginosa. For many bacterial strains, the MICs of all the proteins were found to be at the lowest concentration of 16 μg. Further none of the protein purified showed haemolytic effects on ABO hRBCs.